polyacrylamide gel electrophoresis

Use alkaline lysis method to extract the plasmid DNA. DNA constructs were identified by Pst I/ Bgl II digestion, denaturing polyacrylamide gel electrophoresis and DNA sequence analysis.

随机挑选单菌落小规模培养，碱裂解法提取质粒DNA，PstⅠ／BglⅡ双酶切后行变性聚丙烯酰胺凝胶电泳和银染分析，筛选含有不同等位基因的重组质粒后测序。

互联网摘选

Methods Disc-polyacrylamide gel electrophoresis ( PAGE ) was used to detect serum proteins.

方法圆盘聚丙烯酰胺凝胶电泳 ( PAGE ) 用于血清蛋白检测.

互联网摘选

Testing trueness and purity of potato variety by tuber total protein polyacrylamide gel electrophoresis technique

用马铃薯块茎全蛋白聚丙烯酰胺凝胶电泳技术鉴定品种的真实性和纯度

互联网摘选

Two-dimensional polyacrylamide gel electrophoresis of newt gastrula ectoderm treated by inducing agents

蝾螈原肠胚外胚层诱导处理后蛋白质的双向电泳

互联网摘选

The polymorphism of hemoglobin ( Hb) and transferring ( Tf) of 42 individuals of Minxian Black-fur sheep was detected by using polyacrylamide gel electrophoresis.

采用不连续非变性聚丙烯酰胺凝胶电泳技术对42只岷县黑裘皮羊的血红蛋白（Hb）和转铁蛋白（Tf）基因座的多态性进行了研究。

互联网摘选

The effects of two protein additives, namely, egg white powder and wheat gluten, on the gel properties of bighead surimi were investigated by testing the texture and microstructure of the surimi gels. SDS-polyacrylamide gel electrophoresis ( SDS-PAGE) was used to investigate the corresponding mechanism.

通过检测鱼糜凝胶的质构及微观结构，研究了鸡蛋清蛋白和谷朊粉两种蛋白类添加剂对鳙鱼鱼糜凝胶特性的影响，并采用SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）对相关作用机理进行分析。

互联网摘选

Results ① The self-assembled bDNA is about 500 bp according to the result of diamine silver stain procedure following the polyacrylamide gel electrophoresis ( PAGE).

结果①自行组装的bDNA分子以5%非变性聚丙烯酰胺凝胶银氨染色显示其大约为500bp。

互联网摘选

This paper dealt with polyacrylamide gel electrophoresis ( PAGE ) of lactate dehydrogenase ( LDH ) isoenzymes in fish.

本文对用聚丙烯酰胺凝胶电泳 ( AGE ) 离鱼类乳酸脱氢酶 ( DH ) 工酶的技术进行了研究.

网络文摘精选

This paper studies polyacrylamide gel electrophoresis of rhizobial esterase, superoxide dismutase ( SOD) and glucose ─ 6 ─ phosphate dehydrogenase ( G6PD). Glucose Oxidase ─ ─ A Promising Replacer of Bromate

本文是有关根瘤菌酯酶、超氧物歧化酶（SOD）及葡萄糖6磷酸脱氢酶（66PD）等同工酶的聚丙烯酰胺凝肢电泳及特异性显色判断方法报道。葡萄糖氧化酶&颇具前途的溴酸钾替代品

互联网摘选

Five blood protein loci ( Alb, Pro, Tf, Prt, Hb ) of 40 Xinjiang Tianshan Wapiti were detected by vertical polyacrylamide gel electrophoresis.

采用垂直板聚丙烯酰胺凝胶电泳技术, 分析了40头新疆天山马鹿的血清白蛋白(Alb), 前白蛋白 ( Pro), 转铁蛋白(Tf ),前转铁蛋白(Prt),血红蛋白(Hb)5个蛋白座位的基因型频率 、 基因频率和群体遗传变异指标,以便为天山马鹿选种选配繁育工作提供理论依据.

网络文摘精选

The PCR-PAGE assay uses polyacrylamide gel electrophoresis to determine telomeric repeat amplification products.

PCR-PAGE法是将端粒重复序列PCR产物直接进行聚丙烯酰胺凝胶电泳，通过硝酸银染色测定端粒酶活性。

互联网摘选

Four microsatellite markers linked to FecB locus on 6 chromosome in ovine were analyzed for 108 animals by PCR and denaturing polyacrylamide gel electrophoresis.

选择4个位于绵羊6号染色体上且与FecB基因紧密连锁的微卫星标记，对小尾寒羊基因组进行PCR扩增后，采用最小二乘法估计各等位基因片段对产羔数的影响。

互联网摘选

Method: The genetic polymorphism of 144 unrelated individuals of Hui population and 104 individuals of Han population from Ningxia was analyzed by polymerase chain reaction ( PCR) amplification combined with denaturing polyacrylamide gel electrophoresis ( PAGE) and silver staining.

方法：采用PCR扩增技术和聚丙烯酰胺凝胶电泳方法，分析144名无血缘关系的宁夏回族和104名宁夏汉族健康个体的遗传多态性。

互联网摘选

Methods Immuno affinity chromatography, SDS polyacrylamide gel electrophoresis, immunohistochemistry and immunoelectron microscopy were used.

方法用免疫亲和层析和聚丙烯酰胺凝胶电泳纯化、分析了MSH27识别的小鼠睾丸抗原，并用免疫组织化学和免疫电镜进行了定位。

互联网摘选

Analysis on polyacrylamide gel electrophoresis of serum proteins of Trionyx sinensis

中华鳖血清蛋白聚丙烯酰胺凝胶电泳的初步分析

互联网摘选

The physicochemical and antibacterial properties of antibacterial peptides were verified by using the methods of plate growth inhibition assay, bactericidal assay and belt growth inhibition assay with polyacrylamide gel electrophoresis.

用抑菌圈测定，溶菌活力测定及聚丙酰凝胶电泳抑菌带显示试验的方法分析抗菌肽的抗菌特征和理化特性。

互联网摘选

Methods: By using of preparative acid urea polyacrylamide gel electrophoresis ( AU PAGE), three major HRPs ( HRP 1, HRP 3 and HRP 5) were purified from parotid saliva collected from healthy adults.

方法：采用制备性酸性尿素聚丙烯酰胺凝胶电泳（AU-PAGE），从健康成人刺激性腮腺唾液中分离纯化3种主要富组蛋白（HRP-1、HRP-3、HRP-5）；

互联网摘选

S-protein was isolated from RNase A by polyacrylamide gel electrophoresis afterproteolytic digestion with subtilisin.

核糖核酸酶A具有DNA结合蛋白的作用.

互联网摘选

Determination of Keratin Composition of Discolored Human Hair by Two-Dimensional Polyacrylamide Gel Electrophoresis

聚丙烯酰胺双向凝胶电泳法分析变色人发角蛋白组成

互联网摘选

The experimental steps include extracting DNA by chelex 100, amplification of DNA, polyacrylamide gel electrophoresis, silver staining, DNA typing by standard samples.

采用chelex100快速抽提DNA，DNA扩增，聚丙烯酰胺凝胶电泳、银染色法，参照标准品进行DNA分型。

互联网摘选

The purified biliverdin reductase preparation appeared a single protein band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis which the molecular weight of is about 47,000 dalton.

经SDS&聚丙烯酰胺凝胶电泳表明，该酶达到均一程度，分子量约为47000道尔顿。

互联网摘选

Molecular weight by sodium dodecyl sulphate-polyacrylamide gel electrophoresis was estimated to be 28 700 dalton.

在40℃的磷酸氢二钠—柠檬酸缓冲液中测得该酶的分子量为28700道尔顿.

互联网摘选

Objective: To establish a method for computer analysis of two dimensional polyacrylamide gel electrophoresis ( 2 D PAGE) image in proteome research.

目的：建立蛋白质组研究中双向聚丙烯酰胺凝胶电泳（two－dimensional polyacrylamide gel electrophoresis，2－D PAGE）图谱的计算机分析方法。

互联网摘选

The purified PLAP was identified by polyacrylamide gel electrophoresis ( PAGE) and high performance liquid chromatography ( HPLC).

聚丙烯酰胺凝胶电泳（PAGE）和高压液相层析（HPLC）鉴定其纯度；

互联网摘选

By means of polyacrylamide gel electrophoresis, peroxidase isoenzymes of six species of Acer L. were analyzed.

采用垂直平板聚丙烯酰胺凝胶电泳的方法分析了槭属六种植物的过氧化物酶同工酶.

互联网摘选